An improved ferric chloride test for differentiating Proteus-Providence group from other Enterobacteriaceae.

نویسندگان

  • J SINGER
  • B E VOLCANI
چکیده

It has been found by a number of investigators that cell suspensions and sonic extracts of one species (Proteus vgaris) of the family Enterobacteriaceae contain an enzyme which catalyzes the oxidative deaTnination of most of the i,-amino acids to their corresponding a-keto acids (Bernheim et al., 1935; Uemura, 1942a, b; Uemura, 1944; Stumpf and Green, 1944). Henriksen (1950) utilized this faculty of P. vulgaris to develop a test for differentiating the Proteus group from other enteric organisms. When cell suspensions are incubated with phenylalanine, ferric ions react with the formed phenylpyruvic acid to yield a green color which, however, fades quickly. This study reports a simplification of the above test and its trial with 22 amino acids giving, in some cases, different colors specific to the acid; tryptophan proved most suitable for the detection of the Proteus-Providence group. Preliminary results were reported earlier (Singer, 1953).

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عنوان ژورنال:
  • Journal of bacteriology

دوره 69 3  شماره 

صفحات  -

تاریخ انتشار 1955